The adult human body harbors multipotent stem cells within any tissue. These tissue-resident stem cells mainly exhibit a perivascular location, and after isolation from their tissue of origin and culture in vitro they manifest the characteristics of mesenchymal stem cells (MSCs). Now that MSCs have been shown to be perivascular in vivo, behaving as dynamic releasers of molecules that are essential for maintaining tissue vitality, a novel scenario is emerging. 

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So far, the so called regenerative medicine has been based upon the idea of isolating the stem cells from patients, expand the extracted stem cells ex vivo and transplant them back into the patient with the hope for a biological therapy of incurable diseases. The compelling evidence that tissue-resident pericytes/MSCs not only harbor a multifaceted differentiating potential, but are responsible for the production of molecules that determine and govern our self-healing capabilities, raise the larger question as to whether the chance for an effective regenerative medicine should be more closely related to the efforts of promoting the endogenous self-healing ability by tissue resident stem cells, rather than relying on stem cell transplantation by itself.

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We have shown that human stem cells are sensitive to and can be oriented by magnetic fields and mechanical vibrations towards multiple differentiating lineages and the production of regenerative proteins, responsible

for (i) the reprogramming to a highly efficient pluripotent state, (ii) the prevention and the reversion of cellular aging, (iii) the enhanced survival to inflammatory and oxidative stresses.

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Relying upon the diffusive feature of electromagnetic and acoustic vibrations we have developed regenerative medicine and well-being approaches capable of targeting our tissue resident stem cells where they are, in any tissue and organ, to reprogram these cells into elements capable to maximize our endogenous self-healing potential.

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In Greek mythology, ELPIS was the personification and the spirit of Hope, with the extension to expectation, and finding relief from suffering. With the purpose not only to investigate the molecular basis of cellular dynamics, but to explore Biology in the light of Physics, we have uncovered essential mechanisms underlying the orchestration of cellular rhythms and fates.

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We are affording unprecedented strategies for tissue regeneration, rejuvenation and morphogenesis eliciting these paths from the inside, as endogenous processes (Endogenous REgeneration/REjuvenation, Morphogenesis: EREMO), without the need for stem cell transplantation.

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A major issue we have addressed in Elpis-Eremo is the possibility to optimize cell polarity. Within a healthy organism, somatic and stem cells exhibit a hierarchical assembly and organization of proteins, organelles, ion fluxes, electric fields, DNA, mRNA, microRNA, and microtubuli of the cytoskeleton and nucleoskeleton. This cell polarity is crucial in the physiological modulation of stem cell differentiation and aging, as shown by the fact that altered cell polarization invariantly associates with disease, pathological aging and cancer.

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Overall, the attainment of suitable cell polarity is emerging as a universal attribute for healthy life. We have developed innovative devices affording a fine modulation of (stem) cell polarity at the level of cellular ion fluxes, microtubular dynamics, and chromatin remodeling, disclosing unprecedented perspectives to preserve a delicate intracellular nanotopography and revert substantial traits of stem cell aging and disease.

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Our precision platform devices can be used to promote well-being and self-healing mechanisms at multiple interconnected levels, acting in different conditions of disease, including:

1. Impairment of myocardial contractility, and rhythm

2. Muscle-skeletal impairment: decreased rachis motility, bone demineralization, arthrosis, spinal canal stenosis

3. Decreased organ perfusion: ischemic limbs

4. Tissue scarring

5. Tissue aging: skin senescence (i.e. wrinkles…)

6. Altered proprioception, tremor: Parkinson’s disease, and Alzheimer’s Disease

7. Stress and anxiety

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We can also converge to the treatment of the same conditions by the aid of electromagnetic fields, using defined electromagnetic frequencies, waveforms, pulse duration, and inter-pulse intervals.In Elpis-Eremo, the use of physical energies can be combined with the delivery of arrays of proteins and signaling molecules capable to promote and maintain tissue regeneration, as those provided by human term placenta exosomes, or lyophilized placental extracts.The use of physical energies, alone or in combination with regenerative molecules is also actively pursued and implemented to create novel approaches in the cosmetic field and esthetic medicine.